Reporter

Part:BBa_K786005:Design

Designed by: Leung Wai Tak   Group: iGEM12_Hong_Kong-CUHK   (2012-09-21)


Light triggering gene expression with sensory rhodopsin I & II (SRI & SRII)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2988
    Illegal suffix found in sequence at 940
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2988
    Illegal SpeI site found at 941
    Illegal PstI site found at 955
    Illegal NotI site found at 948
    Illegal NotI site found at 2994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2988
    Illegal XhoI site found at 1972
    Illegal XhoI site found at 2864
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2988
    Illegal suffix found in sequence at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2988
    Illegal XbaI site found at 3003
    Illegal SpeI site found at 941
    Illegal PstI site found at 955
    Illegal AgeI site found at 651
    Illegal AgeI site found at 763
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used strong RBS to allow reporter expression once promoter was activated.


Source

mRFP was from previous biobrick.

References